T-DNA insertional mutagenesis for functional genomics in rice
SCIE
SCOPUS
- Title
- T-DNA insertional mutagenesis for functional genomics in rice
- Authors
- Jeon, JS; Lee, S; Jung, KH; Jun, SH; Jeong, DH; Lee, J; Kim, C; Jang, S; Lee, S; Yang, K; Nam, J; An, K; Han, MJ; Sung, RJ; Choi, HS; Yu, JH; Choi, JH; Cho, SY; Cha, SS; Kim, SI; An, G
- Date Issued
- 2000-06
- Publisher
- BLACKWELL SCIENCE LTD
- Abstract
- We have produced 22 090 primary transgenic rice plants that carry a T-DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel-blot and PCR analyses have shown that approximately 65% of the population contains more than one copy of the inserted T-DNA. Hygromycin resistance tests revealed that transgenic plants contain an average of 1.4 loci of T-DNA inserts. Therefore, it can be estimated that approximately 25 700 taggings have been generated. The binary vector used in the insertion contained the promoterless beta-glucuronidase (GUS) reporter gene with an intron and multiple splicing donors and acceptors immediately next to the right border. Therefore, this gene trap vector is able to detect a gene fusion between GUS and an endogenous gene, which is tagged by T-DNA. Histochemical GUS assays were carried out in the leaves and roots from 5353 lines, mature flowers from 7026 lines, and developing seeds from 1948 lines. The data revealed that 1.6-2.1% of tested organs were GUS-positive in the tested organs, and that their GUS expression patterns were organ- or tissue-specific or ubiquitous in all parts of the plant. The large population of T-DNA-tagged lines will be useful for identifying insertional mutants in various genes and for discovering new genes in rice.
- Keywords
- GLUCURONIDASE GENE FUSIONS; BETA-GLUCURONIDASE; AGROBACTERIUM-TUMEFACIENS; ARABIDOPSIS-THALIANA; ENHANCER TRAP; ELEMENTS; TRANSFORMATION; INTEGRATION; TOBACCO; PLANTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19954
- DOI
- 10.1046/j.1365-313x.2000.00767.x
- ISSN
- 0960-7412
- Article Type
- Article
- Citation
- PLANT JOURNAL, vol. 22, no. 6, page. 561 - 570, 2000-06
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.