Carbachol-induced phosphorylation of phospholipase D1 through protein kinase C is required for the activation in COS-7 cells
SCIE
SCOPUS
- Title
- Carbachol-induced phosphorylation of phospholipase D1 through protein kinase C is required for the activation in COS-7 cells
- Authors
- Lee, BD; Kim, Y; Han, JM; Suh, PG; Ryu, SH
- Date Issued
- 2001-03-31
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Abstract
- Phospholiapse D (PLD), and phosphatidic acid generated by it, have been implicated in receptor-mediated intracellular signaling, Carbachol (CCh) is known to activate PLD1, and protein kinase C (PKC) is known to mediate in this signaling pathway. In recent reports (Kim et al,, 1999b; Kim et al,, 2000), we published our observations of the direct phosphorylation of PLD1 by PKC and we described the phosphorylation-dependent regulation of PLD1 activity. In this study, we investigated the phosphorylation and compartmentalization of PLD1 in terms of CCh signaling in M3 muscarinic receptor (M3R)-expressing COS-7 cells. CCh treatment of COS-7 cells transiently coexpressing PLD1 and M3R stimulated PLD1 activity and induced direct phosphorylation of PLD1 by PKC, The CCh-induced activation and phosphorylation of PLD1 was completely blocked upon pretreatment of the cells with PKC-specific inhibitors. We looked at the localization of the PLD1 phosphorylation by PKC and found that PLD1 was mainly located in the caveolin-enriched membrane (CEM) fraction. Based on these results, we conclude that CCh induces the activation and phosphorylation of PLD1 via PKC and that the phosphorylation of PLD1 occurs in caveolae.
- Keywords
- carbachol; caveolae; M3 muscarinic receptor; phospholipase D1; protein kinase C; MUSCARINIC ACETYLCHOLINE-RECEPTOR; ADP-RIBOSYLATION-FACTOR; PLASMA-MEMBRANE; SIGNAL-TRANSDUCTION; MDCK CELLS; IN-VIVO; CAVEOLAE; ALPHA; RAT; PATHWAY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19618
- ISSN
- 1225-8687
- Article Type
- Article
- Citation
- JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 34, no. 2, page. 182 - 187, 2001-03-31
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