Characterization of MADS box genes from hot pepper
SCIE
SCOPUS
KCI
- Title
- Characterization of MADS box genes from hot pepper
- Authors
- Sung, SK; Moon, YH; Chung, JE; Lee, SY; Park, HG; An, G
- Date Issued
- 2001-06-30
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Abstract
- The cDNA clone, CanMADS1, was isolated from young flower buds of the hot pepper (Capsicum annuum L. by screening a cDNA library using the OsMADS1 rice MADS-bos gene as a probe. We used a yeast two-hybrid screening method to investigate interaction partners of the protein product of CanMADS1. A MAI)S-box gene, CanMADS6, was isolated from young flower buds using the region containing the K domain and 15 amino acid residues of the C-terminal region of CanMADS1 as a bait. CanMADS1 and CanMADS6 showed high amino acid sequence similarities to members of the AGL2 subfamily and the SQUA subfamily, respectively, CanMADS1 transcript was expressed in flower buds and fruits, and the transcription signal was the strongest in the stage of the fruit set (2 d after anthesis). CanMADS6 showed the same expression pattern as CanMADS1. CanMADS1 and CanMADS6 were not expressed in leaves. These results suggest that a regulatory role for flower and fruit development of the hot pepper may be accomplished through an interaction of the protein products of the two MADS-box genes, CanMADS1 and CanMADS6.
- Keywords
- fruit development; hot pepper; MADS genes; yeast two-hybrid; HOMEOTIC PROTEINS APETALA1; DNA-BINDING; ANTIRRHINUM-MAJUS; TRANSCRIPTION FACTORS; REGULATED EXPRESSION; ARABIDOPSIS; FAMILY; PISTILLATA; DOMAIN; IDENTITY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19495
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 11, no. 3, page. 352 - 359, 2001-06-30
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.