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Transgenic radish (Raphanus sativus L. longipinnatus Bailey) by floral-dip method - plant development and surfactant are important in optimizing transformation efficiency SCIE SCOPUS

Title
Transgenic radish (Raphanus sativus L. longipinnatus Bailey) by floral-dip method - plant development and surfactant are important in optimizing transformation efficiency
Authors
Curtis, ISNam, HG
Date Issued
2001-08
Publisher
KLUWER ACADEMIC PUBL
Abstract
Transgenic radish (Raphanus sativus L. longipinnatus Bailey) plants were produced from the progeny of plants which were dipped into a suspension of Agrobacterium carrying both the beta -glucuronidase (gusA) gene and a gene for resistance to the herbicide Basta (bar) between T-DNA border sequences. The importance of development of the floral-dipped plant and presence of surfactant in the inoculation medium were evaluated in terms of transgenic plant production. Plants dipped at the primary bolt stage of growth, into a suspension of Agrobacterium containing 0.05% (v/v) Silwet L-77 resulted in optimum transformation efficiency, with 1.4% from 1110 seeds. The presence of Pluronic F-68 or Tween 20 in the inoculation medium was beneficial towards transgenic plant output compared to treatments without surfactant. Putative transformed T1 plants were efficiently selected by spraying with 0.03% (v/v) Basta and all herbicide-resistant plants tested positive for GUS activity when analysed both histochemically and fluorometrically. Southern analysis revealed that both the gusA and bar genes integrated into the genome of transformed plants and segregated as dominant Mendelian traits. These results demonstrate that radish can be genetically modified for the improvement of this important vegetable crop.
Keywords
Agrobacterium-mediated transformation; floral-dipping; GUS expression; herbicide resistance; Raphanus sativus; transgenic radish; AGROBACTERIUM-MEDIATED TRANSFORMATION; ARABIDOPSIS-THALIANA; BAR GENE; HERBICIDE-RESISTANCE; SHOOT REGENERATION; INFILTRATION; TUMEFACIENS; LETTUCE; CULTURE; MARKER
URI
https://oasis.postech.ac.kr/handle/2014.oak/19456
DOI
10.1023/A:1016600517293
ISSN
0962-8819
Article Type
Article
Citation
TRANSGENIC RESEARCH, vol. 10, no. 4, page. 363 - 371, 2001-08
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남홍길NAM, HONG GIL
Div of Integrative Biosci & Biotech
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