Investigation and interpretation of band broadening in size exclusion chromatography
SCIE
SCOPUS
- Title
- Investigation and interpretation of band broadening in size exclusion chromatography
- Authors
- Busnel, JP; Foucault, F; Denis, L; Lee, W; Chang, T
- Date Issued
- 2001-09-28
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Study of Band Broadening occurring in Size Exclusion Chromatography (SEC) is reported using very narrow PS standards obtained and characterised by Temperature Gradient Interaction Chromatography (TGIC). Chromatograms are fitted by Exponentially Modified Gaussian functions (EMG) and mapping of band broadening is obtained for different column sets. Interpretation of the skewing of the chromatograms is proposed with a new model using Brownian motion properties inside the pores. That explains why band broadening and tailing become so important near total exclusion volume. (C) 2001 Elsevier Science B.V. All rights reserved.
- Keywords
- band broadening; temperature-gradient interaction chromatography; peak shape; exponentially modified Gaussian functions; GRADIENT INTERACTION CHROMATOGRAPHY; POLYSTYRENE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19360
- DOI
- 10.1016/S0021-9673(01)01159-1
- ISSN
- 0021-9673
- Article Type
- Article
- Citation
- JOURNAL OF CHROMATOGRAPHY A, vol. 930, no. 1-2, page. 61 - 71, 2001-09-28
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