Negative regulatory role of overexpression of PLC gamma 1 in the expression of early growth response 1 gene in rat 3Y1 fibroblasts
SCIE
SCOPUS
- Title
- Negative regulatory role of overexpression of PLC gamma 1 in the expression of early growth response 1 gene in rat 3Y1 fibroblasts
- Authors
- Shin, SY; Ko, JS; Chang, JS; Min, DS; Choi, C; Bae, SS; Kim, MJ; Hyun, DS; Kim, JH; Han, MY; Kim, YH; Kim, YS; Na, DS; Suh, PG; Lee, YH
- Date Issued
- 2002-10
- Publisher
- FEDERATION AMER SOC EXP BIOL
- Abstract
- The early growth response 1 (Egr-1) gene product is a transcription factor that functions as an oikis factor. Loss of Egr-1 expression is closely associated with tumor formation. Phospholipase Cgammal (PLCgamma1) is overexpressed in some tumors, and its overexpression causes anchorage-independent growth. Here we report that overexpression of PLCgammal and SH2-SH3 domain of PLCgamma1 decreased induction of Egr-1 and the Egr-1-regulated genes TSP-1 and PAI-1. Results from the nuclear run-on assay and transfection experiment with the proximal 455 base pair region of the Egr-1 promoter (-454 to +1) showed that Egr-1 transcriptional activity was suppressed in PLCgamma1-3Y1 cells whereas decay of Egr-1 mRNA was similar in both cell lines. Serum response element- and ternary complex factor Elk-l-mediated transcriptional activation of the reporter gene in response to EGF were also inhibited in PLCgamma1-3Y1 cells. Pretreatment with the protein synthesis inhibitor cycloheximide (CHX) partially abrogated the serum-induced suppression of Egr-1 transcription in PLCgammal-3Y1 cells, suggesting that a CHX-sensitive factor(s) is involved in the suppression of Egr-1 transcription in PLCgamma1-3Y1 cells. Our results demonstrated that overexpression of PLCgamma1 functions as a negative modulator of the tumor suppressor Egr-1 gene expression, possibly through inhibition of Elk-l-dependent transcriptional activity.
- Keywords
- Egr-1; tumor suppressor; PLG gamma 1 overexpression; TRANSCRIPTION FACTOR EGR-1; TERNARY COMPLEX FACTORS; PROTEIN-SYNTHESIS INHIBITORS; FIBROSARCOMA HT1080 CELLS; COLONY-STIMULATING FACTOR; PHOSPHOLIPASE C-GAMMA-1; C-FOS; TRANSFORMING GROWTH-FACTOR-BETA-1; DIFFERENTIAL REGULATION; ELEVATED CONTENT
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18836
- DOI
- 10.1096/fj.02-0022com
- ISSN
- 0892-6638
- Article Type
- Article
- Citation
- FASEB JOURNAL, vol. 16, no. 12, page. 1504 - 1514, 2002-10
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.