Phospholipase C-gamma is required for agonist-induced Ca2+ entry
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SCOPUS
- Title
- Phospholipase C-gamma is required for agonist-induced Ca2+ entry
- Authors
- Patterson, RL; van Rossum, DB; Ford, DL; Hurt, KJ; Bae, SS; Suh, PG; Kurosaki, T; Snyder, SH; Gill, DL
- Date Issued
- 2002-11-15
- Publisher
- CELL PRESS
- Abstract
- We report here that PLC-gamma isoforms are required for agonist-induced Ca2+ entry (ACE). Overexpressed wild-type PLC-gamma1 or a lipase-inactive mutant PLC-gamma1 each augmented ACE in PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of PLC-gamma1 was ineffective. RNA interference to deplete either PLC-gamma1 or PLC-gamma2 in PC12 and A7r5 cells inhibited ACE. In DT40 B lymphocytes expressing only PLC-gamma2, overexpressed muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC-gamma2 knockout cells, M5R stimulation of ER Ca2+ store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of PLC-gamma2 or a lipase-inactive PLC-gamma2 mutant. The results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry.
- Keywords
- CAPACITATIVE CALCIUM-ENTRY; OPERATED HTRP3 CHANNELS; NECROTIC CELL-DEATH; TYROSINE PHOSPHORYLATION; ENDOPLASMIC-RETICULUM; STORE DEPLETION; TRPC3 CHANNELS; RECEPTOR; PROTEIN; ACTIVATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18819
- DOI
- 10.1016/S0092-8674(02)01045-0
- ISSN
- 0092-8674
- Article Type
- Article
- Citation
- CELL, vol. 111, no. 4, page. 529 - 541, 2002-11-15
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- There are no files associated with this item.
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