Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries
SCIE
SCOPUS
- Title
- Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries
- Authors
- Chung, J; Park, S; Kim, D; Rhim, J; Kim, I; Choi, I; Yi, K; Ryu, S; Suh, P; Chung, D; Bae, Y; Shin, Y; Park, SH
- Date Issued
- 2002-12
- Publisher
- SPRINGER-VERLAG
- Abstract
- Purpose: In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized. Methods: From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mAb through repeated rounds of panning on a mAb-coated ELISA plate. The antigenicity and immunogenicity of the peptide epitopes were then studied using chemically synthesized peptides. Results: The selected clones had the LXPSIP consensus sequence. Two synthetic peptides LPPSIPFGLTVGGGGS and LLPSIPNQAYLGGGGS specifically reacted with anti-JL1 mAb in ELISA. These two peptides were found to inhibit the interaction between anti-JL1 mAb and JL1 antigen-positive Molt-4 cells. Although the immune sera raised against the keyhole limpet hemocyanin-conjugated peptides failed to react with Molt-4 cells, it showed strong reactivity to the peptide epitope. However, one mAb raised by peptide immunization successfully bound to Molt-4 cells. Conclusion: An epitope-mimetic peptide of anti-JL1 mAb was found using combinatorial peptide phage display libraries. It induced strong humoral response against itself, but only a limited fraction of this humoral response was cross-reactive with the original JL1 antigen.
- Keywords
- phage display; combinatorial peptide library; mimotope-monoclonal antibody; JL1 antigen; DISCONTINUOUS EPITOPES; JL1; MIMOTOPES; IMMUNIZATION; GENERATION; INHIBITION; EXPRESSION; THYMOCYTE; MIMICKING; MOLECULE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18769
- DOI
- 10.1007/s00432-002-0390-x
- ISSN
- 0171-5216
- Article Type
- Article
- Citation
- JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 128, no. 12, page. 641 - 649, 2002-12
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