Thiram and ziram stimulate non-selective cation channel and induce apoptosis in PC12 cells
SCIE
SCOPUS
- Title
- Thiram and ziram stimulate non-selective cation channel and induce apoptosis in PC12 cells
- Authors
- Han, MS; Shin, KJ; Kim, YH; Kim, SH; Lee, T; Kim, E; Ryu, SH; Suh, PG
- Date Issued
- 2003-06
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- The neurotoxicity of dithiocarbamates has been previously reported, however the detailed mechanism underlying the neurotoxicity is still not fully understood Among the dithiocarbamates, we investigated thiram and ziram in a neuronal-like pheochromocytoma (PC12) cells. Thiram and ziram strongly induced cell death in both dose- and time-dependent manners with the LC50 of 0.3 and 2 muM, respectively. The cell death showed typical apoptotic features, such as DNA fragmentation and an increase of subdiploidy nuclei. Interestingly, both thiram and ziram induced rapid and sustained increases of intracellular Ca2+ in PC12 cells, which were almost completely blocked by flufenamic acid (FFA), an inhibitor of non-selective cation channel. BAPTA-AM, an intracellular Ca2+ chelator inhibited the thiram- and ziraminduced apoptotic cell death. These results suggest that thiram and ziram induce apoptotic neuronal cell death by Ca2+ influx through non-selective cation channels. The present study may provide a clue for understanding the mechanism of neurotoxicity of thiram and ziram. (C) 2003 Published by Elsevier Science Inc.
- Keywords
- thiram; ziram; zineb; apoptosis; PC12 cells; non-selective cation channel; ENDOTHELIAL-CELLS; DITHIOCARBAMATE; CA2+; RAT; FUNGICIDES; GENOTOXICITY; ACTIVATION; TOXICITY; DISEASE; INVITRO
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18495
- DOI
- 10.1016/S0161-813X(03)00013-5
- ISSN
- 0161-813X
- Article Type
- Article
- Citation
- NEUROTOXICOLOGY, vol. 24, no. 3, page. 425 - 434, 2003-06
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