Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1-induced MEK/ERK phosphorylation and migration in endothelial cells
SCIE
SCOPUS
- Title
- Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1-induced MEK/ERK phosphorylation and migration in endothelial cells
- Authors
- Yoon, M; Cho, CH; Lee, CS; Jang, IH; Ryu, SH; Koh, GY
- Date Issued
- 2003-08-15
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Angiopoietin-1 (Ang1) and its receptor, Tie2, play critical roles in blood vessel formation. Ang1 triggers a variety of signaling events in endothelial cells leading to vasculogenic and angiogenic processes. However, the underlying mechanism for Ang1/Tie2 signaling is not fully understood. Here, we show that Tie2 and phospholipase D (PLD) are localized in the caveolae, specialized subdomains of the endothelial cell plasma membrane enriched with signaling molecules. Interestingly, Ang1 increased PLD activities in a dose- and time-dependent manner. Ang1-induced MEK/ERK activation was abrogated when PLD was inhibited, suggesting that PLD mediates Ang1-induced MEK/ERK activation. Moreover, PLD inhibitor, 1-butanol, inhibited Ang1-induced endothelial cell migration. Our results indicate that: (1) caveolae may be the platform for Tie2/PLD association in endothelial cells; (2) PLD is a new mediator of Ang1/Tie2-induced signaling pathway, and it participates in MAPK activation and endothelial cell migration. (C) 2003 Elsevier Inc. All rights reserved.
- Keywords
- SIGNAL-TRANSDUCTION; GROWTH-FACTORS; LIPID RAFTS; ACTIVATION; SURVIVAL; TRANSLOCATION; EXPRESSION; CLONING; PROTEIN; BINDING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18416
- DOI
- 10.1016/S0006-291X(03)01341-X
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 308, no. 1, page. 101 - 105, 2003-08-15
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