Luteolin inhibits the nuclear factor-kappa B transcriptional activity in Rat-1 fibroblasts
SCIE
SCOPUS
- Title
- Luteolin inhibits the nuclear factor-kappa B transcriptional activity in Rat-1 fibroblasts
- Authors
- Kim, SH; Shin, KJ; Kim, D; Kim, YH; Han, MS; Lee, TG; Kim, E; Ryu, SH; Suh, PG
- Date Issued
- 2003-09-15
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Abstract
- Flavonoids are natural polyphenolic compounds that have anti-inflammatory, cytoprotective and anticarcinogenic effects. In this study, we investigated the effects of several flavonoids on nuclear factor-kappa B (NF-kappaB) activation by using luciferase reporter gene assay. Among the flavonoids examined, luteolin showed the most potent inhibition on lipopolysaccharide (LPS)-stimulated NF-kappaB transcriptional activity in Rat-1 fibroblasts. Luteolin did not inhibit either IkappaBalpha. degradation or NF-kappaB nuclear translocation, DNA binding or phosphorylation by LPS. However, luteolin prevented LPS-stimulated interaction between the p65 subunit of NF-kappaB and the transcriptional coactivator CBP. In addition, a specific PKA inhibitor that blocked the phosphorylation of CREB and c-Jun by luteolin partially reversed the inhibitory effect of luteolin on NF-kappaB(.)CBP complex formation and NF-kappaB transcriptional activity by LPS. These data imply that inhibition of NF-kappaB transcriptional activity by luteolin may occur through competition with transcription factors for coactivator that is available in limited amounts. Taken together, this study provides a molecular basis for the understanding of the antiinflammatory effects of luteolin. (C) 2003 Elsevier Inc. All rights reserved.
- Keywords
- nuclear factor-kappa B; luteolin; coactivator; lipopolysaccharide; fibroblast; anti-inflammation; CBP-INDUCED STIMULATION; MEDIATED TRANSCRIPTION; PROTEIN CBP; IN-VIVO; FLAVONOIDS; ACTIVATION; COACTIVATOR; CREB; PHOSPHORYLATION; INFLAMMATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18329
- DOI
- 10.1016/S0006-2952(03)00465-9
- ISSN
- 0006-2952
- Article Type
- Article
- Citation
- BIOCHEMICAL PHARMACOLOGY, vol. 66, no. 6, page. 955 - 963, 2003-09-15
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