Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction
SCIE
SCOPUS
- Title
- Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction
- Authors
- Park, N; Kim, S; Hahn, JH
- Date Issued
- 2003-11-01
- Publisher
- AMER CHEMICAL SOC
- Abstract
- A compact, thermal-cycling device for high-throughput continuous-flow polymerase chain reaction (PCR) has been developed, which consists of a flow-through capillary and a cylindrical heating-block assembly. A 3.5-m-long fused-silica capillary coils helically, with 33 turns, up around the 30-mm-diameter assembly of three equally divided thermostating copper blocks for melting, annealing, and extension. An injected PCR mixture undergoes one cycle of PCR each turn. A continuous-flow PCR of one sample and also a segmented-flow PCR of four different samples have been successfully demonstrated. The present device can easily evolve into a parallel-processing, multistation compact device and be modified to have real-time PCR capability. Ibis solid-based compact PCR device, therefore, has a potentiality to be the format of choice when developed for a portable system.
- Keywords
- DNA AMPLIFICATION; PCR AMPLIFICATION; SYSTEMS; ELECTROPHORESIS; MICROCHIP; CHAMBERS; CHIPS; TIME
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18257
- DOI
- 10.1021/AC0346959
- ISSN
- 0003-2700
- Article Type
- Article
- Citation
- ANALYTICAL CHEMISTRY, vol. 75, no. 21, page. 6029 - 6033, 2003-11-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.