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A complete sequence of the pGA1611 binary vector SCIE KCI

Title
A complete sequence of the pGA1611 binary vector
Authors
Kim, SRLee, SKang, HGJeon, JSKim, KMAn, GH
Date Issued
2003-09-30
Publisher
BOTANICAL SOCIETY OF KOREA
Abstract
We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577-bp T-DNA region consists of a 1987-bp ubiquitine promoter region, 45 bp for the multiple cloning site, a 253-bp nos terminator region, and the 2045-bp CaMV35S-hph-T7 chimaeric gene. The vector backbone (7004 bp) carries oriT, traJ, trfA, teM, tetR, and oriV An 892-bp RB region and the 489-bp LB region are also present. The T-DNA possesses 15 unique sites, six of which are at the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector.
Keywords
binary vector; pGA1611; rice; sequence; transformation; DNA INSERTIONAL MUTAGENESIS; T-DNA; AGROBACTERIUM-TUMEFACIENS; TRANSGENIC PLANTS; NUCLEOTIDE-SEQUENCE; RICE PLANTS; GENE; TRANSFORMATION; EXPRESSION; SYSTEM
URI
https://oasis.postech.ac.kr/handle/2014.oak/18189
DOI
10.1007/BF03030451
ISSN
1226-9239
Article Type
Article
Citation
JOURNAL OF PLANT BIOLOGY, vol. 46, no. 3, page. 211 - 214, 2003-09-30
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안진흥AN, GYNHEUNG
Div of Integrative Biosci & Biotech
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