A complete sequence of the pGA1611 binary vector
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- Title
- A complete sequence of the pGA1611 binary vector
- Authors
- Kim, SR; Lee, S; Kang, HG; Jeon, JS; Kim, KM; An, GH
- Date Issued
- 2003-09-30
- Publisher
- BOTANICAL SOCIETY OF KOREA
- Abstract
- We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577-bp T-DNA region consists of a 1987-bp ubiquitine promoter region, 45 bp for the multiple cloning site, a 253-bp nos terminator region, and the 2045-bp CaMV35S-hph-T7 chimaeric gene. The vector backbone (7004 bp) carries oriT, traJ, trfA, teM, tetR, and oriV An 892-bp RB region and the 489-bp LB region are also present. The T-DNA possesses 15 unique sites, six of which are at the multiple cloning site. This information will be valuable for cloning foreign DNA and modifying the vector.
- Keywords
- binary vector; pGA1611; rice; sequence; transformation; DNA INSERTIONAL MUTAGENESIS; T-DNA; AGROBACTERIUM-TUMEFACIENS; TRANSGENIC PLANTS; NUCLEOTIDE-SEQUENCE; RICE PLANTS; GENE; TRANSFORMATION; EXPRESSION; SYSTEM
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18189
- DOI
- 10.1007/BF03030451
- ISSN
- 1226-9239
- Article Type
- Article
- Citation
- JOURNAL OF PLANT BIOLOGY, vol. 46, no. 3, page. 211 - 214, 2003-09-30
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