NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi
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- Title
- NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi
- Authors
- Yoo, JC; Chang, JR; Kim, SH; Jang, SK; Wolgemuth, DJ; Kim, K; Rhee, K
- Date Issued
- 2004-01-15
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Nek2 is a mammalian protein kinase structurally homologous to Aspergillus NIMA. We previously observed that the Nek2 protein was localized in multiple sites within a cell in a cell cycle stage-specific manner. Such dynamic behavior of Nek2 allowed us to propose that Nek2 may be a mitotic regulator that is involved in diverse cell cycle events. To better understand the cellular processes in which Nek2 participates, we carried out yeast two-hybrid screening and isolated Nek2-Interacting Protein 1 (NIP1), which has been also named as XB51 and NECAB3. Physical interactions of Nek2 with NIP1 were confirmed. In fact, Nek2 can phosphorylate NIP1 in vivo. Immumostaining experiments revealed that NIP I is a Golgi protein. These results propose a possible involvement of Nek2 in biological processes of the Golgi body, perhaps in relation to the inheritance of Golgi during mitosis or to cell cycle stage-specific regulation of exocytosis. (C) 2003 Elsevier Inc. All rights reserved.
- Keywords
- Nek2; cell cycle; XB51; NECAB3; NIP1; Golgi; mitosis; AMYLOID PRECURSOR PROTEIN; POLO-LIKE KINASE; CELL-CYCLE; CHROMOSOME SEGREGATION; MITOSIS; CENTROSOME; NIMA; PHOSPHORYLATION; FRAGMENTATION; REGULATOR
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18165
- DOI
- 10.1016/j.yexcr.2003.09.025
- ISSN
- 0014-4827
- Article Type
- Article
- Citation
- EXPERIMENTAL CELL RESEARCH, vol. 292, no. 2, page. 393 - 402, 2004-01-15
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