Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents
SCIE
SCOPUS
- Title
- Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents
- Authors
- Han, MS; Jung, SO; Kim, MJ; Kim, DH
- Date Issued
- 2004-04-16
- Publisher
- AMER CHEMICAL SOC
- Abstract
- A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.
- Keywords
- THROUGHPUT-SCREENING METHOD; METHYL-BETA-CYCLODEXTRIN; ENANTIOSELECTIVE CATALYSTS; ENANTIOMERIC EXCESS; ENZYME-ACTIVITY; BIOCATALYSTS; ACTIVATION; SUBTILISIN; HYDROLASES; CRYSTALS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17982
- DOI
- 10.1021/JO040104I
- ISSN
- 0022-3263
- Article Type
- Article
- Citation
- JOURNAL OF ORGANIC CHEMISTRY, vol. 69, no. 8, page. 2853 - 2855, 2004-04-16
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.