Phytoestrogen cimicifugoside-mediated inhibition of catecholamine secretion by blocking nicotinic acetylcholine receptor in bovine adrenal chromaffin cells
SCIE
SCOPUS
- Title
- Phytoestrogen cimicifugoside-mediated inhibition of catecholamine secretion by blocking nicotinic acetylcholine receptor in bovine adrenal chromaffin cells
- Authors
- Woo, KC; Park, YS; Jun, DJ; Lim, JO; Baek, WY; Suh, BS; Kim, KT
- Date Issued
- 2004-05-01
- Publisher
- AMER SOC PHARMACOLOGY EXPERIMENTAL TH
- Abstract
- We investigated the effect of the phytoestrogen cimicifugoside, one of the pharmacologically active ingredients of the medicinal plant Cimicifuga racemosa (black cohosh) that has been used to treat many kinds of neuronal and menopausal symptoms, such as arthritis, menopausal depression, and nerve pain. Cimicifugoside inhibited calcium increase induced by 1,1-dimethyl-4-phenylpiperazinium iodide ( DMPP), a nicotinic acetylcholine receptor ( nAChR) agonist in bovine adrenal chromaffin cells with a half-maximal inhibitory concentration (IC50) of 18 +/- 2 muM. In contrast, cimicifugoside did not affect the calcium increases evoked by high K+, veratridine, and bradykinin. The DMPP-induced sodium increase was also inhibited by cimicifugoside with an IC50 of 2 +/- 0.3 muM, suggesting that the activity of nAChRs is inhibited by cimicifugoside. Cimicifugoside did not affect the KCI-induced secretion but markedly inhibited the DMPP-induced catecholamine secretion that was monitored by carbon-fiber amperometry in real time and high-performance liquid chromatography through electrochemical detection. The results suggest that cimicifugoside selectively inhibits nAChR-mediated response in bovine chromaffin cells.
- Keywords
- PERFORMANCE LIQUID-CHROMATOGRAPHY; STEROID-HORMONES; CALCIUM-CHANNELS; MEDULLA CELLS; PC12 CELLS; METABOLITES; ESTROGENS; 17-BETA-ESTRADIOL; FLUORESCENCE; GENERATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17971
- DOI
- 10.1124/jpet.103.062331
- ISSN
- 0022-3565
- Article Type
- Article
- Citation
- JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, vol. 309, no. 2, page. 641 - 649, 2004-05-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.