Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OFOVEREXPRESSION OFCO 1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice
SCIE
SCOPUS
- Title
- Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OFOVEREXPRESSION OFCO 1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice
- Authors
- Lee, SY; Kim, J; Han, JJ; Han, MJ; An, GH
- Date Issued
- 2004-06
- Publisher
- BLACKWELL PUBLISHING LTD
- Abstract
- A late-flowering mutant was isolated from rice T-DNA-tagging lines. T-DNA had been integrated into the K-box region of Oryza sativa MADS50 (OsMADS50), which shares 50.6% amino acid identity with the Arabidopsis MADS-box gene SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/AGL20). While overexpression of OsMADS50 caused extremely early flowering at the callus stage, OsMADS50 RNAi plants exhibited phenotypes of late flowering and an increase in the number of elongated internodes. This confirmed that the phenotypes observed in the knockout (KO) plants are because of the mutation in OsMADS50. RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O. sativa GIGANTEA (OsGI). These results suggest that OsMADS50 is an important flowering activator that controls various floral regulators in rice.
- Keywords
- flowering; MADS; OsMADS50; rice; SOC1/AGL20; T-DNA; MADS-BOX GENE; QUANTITATIVE TRAIT LOCUS; ORYZA-SATIVA L.; CONTROLLING HEADING DATE; SHORT-DAY PLANT; PHOTOPERIOD SENSITIVITY; ECTOPIC EXPRESSION; EPISTATIC INTERACTIONS; CULTIVATED RICE; TRANSGENIC RICE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17937
- DOI
- 10.1111/j.1365-313X.2004.02082.x
- ISSN
- 0960-7412
- Article Type
- Article
- Citation
- PLANT JOURNAL, vol. 38, no. 5, page. 754 - 764, 2004-06
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.