Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4
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- Title
- Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4
- Authors
- Irino, Y; Cho, HY; Nakamura, Y; Nakahara, M; Furutani, M; Suh, PG; Takenawa, T; Fukami, K
- Date Issued
- 2004-07-23
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, beta-, gamma-, delta-, epsilon- and zeta-type. PLCdelta-type is reported to be composed of four isozymes, PLCdelta1-delta4. Here we report that a screening for mouse PLCdelta2 from a BAC library with primers that amplify a specific region of bovine PLCdelta2 resulted in isolation of one clone containing the mouse PLCdelta4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCdelta2 and PLCdelta4 in the mouse and human genomes, indicating that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4. However, PLCdelta2 Western blot analysis with a widely used commercial anti-PLCdelta2 antibody showed all expression pattern distinct from that of PLCdelta4 in wildtype mice. In addition, an 80-kDa band, which was recognized by antibody against PLCdelta2, was smaller than an 85-kDa band detected by anti-PLCdelta4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCdelta4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCdelta2 antibody contained no PLC activity. From these data, we conclude that bovine PLCdelta2 is a homologue of human and mouse PLCdelta4, and that three isozymes (delta1, delta3, and delta4) exist in the PLCdelta family. (C) 2004 Elsevier Inc. All rights reserved.
- Keywords
- phospholipase C; PLC delta 2; PLC delta 4; mouse genome; INOSITOL TRISPHOSPHATE; SIGNAL-TRANSDUCTION; HYPERTENSIVE RATS; ACROSOME REACTION; MEMBRANE; CELL; DOMAIN; EXPRESSION; C-DELTA-4; EPSILON
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17830
- DOI
- 10.1016/J.BBRC.2004.
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 320, no. 2, page. 537 - 543, 2004-07-23
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