Biodegradation of dibenzo-p-dioxin, dibenzofuran, and chlorodibenzo-p-dioxins by Pseudomonas veronii PH-03
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SCOPUS
- Title
- Biodegradation of dibenzo-p-dioxin, dibenzofuran, and chlorodibenzo-p-dioxins by Pseudomonas veronii PH-03
- Authors
- Hong, HB; Nam, IH; Murugesan, K; Kim, YM; Chang, YS
- Date Issued
- 2004-10
- Publisher
- KLUWER ACADEMIC PUBL
- Abstract
- The dioxin-degrading strain Pseudomonas veronii PH-03 was isolated from contaminated soil by selective enrichment techniques. Strain PH-03 grew on dibenzo-p-dioxin and dibenzofuran as a sole carbon source. Further, 1-chlorodibenzo-p-dioxin, 2-chlorodibenzo-p-dioxin and other dioxin metabolites, salicylic acid, and catechol were also metabolized well. Resting cells of strain PH-03 transformed dibenzo-p-dioxin, dibenzofuran, 2,2,3-trihydroxybiphenyl, and some chlorodioxins to their corresponding metabolic intermediates such as catechol, salicylic acid, 2-hydroxy-(2-hydroxyphenoxy)-6-oxo-2,4-hexadienoic acid, and chlorocatechols. The formation of these metabolites was confirmed by comparison of gas chromatography-mass spectrometry (GC-MS) data with those of authentic compounds. Although we did observe the production of 3,4,5,6-tetrachlorocatechol (3,4,5,6-TECC) from 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TCDD) with resting cell suspensions of PH-03, growth of strain PH-03 in the presence of 1,2,3,4-TCDD was poor. This result suggests that strain PH-03 is unable to utilize 3,4,5,6-TECC, even at very low concentration (0.01 mM) due to its toxicity. In cell-free extracts of DF-grown cells, 2,2',3-trihydroxybiphenyl dioxygenase, 2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid hydrolase, and catechol-2,3-dioxygense activities were detected. Moreover, the activities of meta-pyrocatechase and 2,2',3-trihydroxybiphenyl dioxygenase from the crude cell-free extracts were inhibited by 3-chlorocatechol. However, no inhibition was observed in intact cells when 3-chlorocatechol was formed as intermediate.
- Keywords
- biodegradation; chlorodibenzo-p-dioxins; dibenzofuran; dibenzo-p-dioxin; Pseudomonas veronii PH-03; SP STRAIN RW1; MICROBIAL-DEGRADATION; CHLORINATED DIBENZOFURANS; ANGULAR DIOXYGENATION; DIARYL ETHERS; PUTIDA GJ31; METABOLISM; PURIFICATION; BACTERIUM; 2,2' ,3-TRIHYDROXYBIPHENYL
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17649
- DOI
- 10.1023/B:BIOD.0000042185.04905.0d
- ISSN
- 0923-9820
- Article Type
- Article
- Citation
- BIODEGRADATION, vol. 15, no. 5, page. 303 - 313, 2004-10
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