A brain-targeted rabies virus glycoprotein-disulfide linked PEI nanocarrier for delivery of neurogenic microRNA
SCIE
SCOPUS
- Title
- A brain-targeted rabies virus glycoprotein-disulfide linked PEI nanocarrier for delivery of neurogenic microRNA
- Authors
- Hwang, DW; Son, S; Jang, J; Youn, H; Lee, S; Lee, D; Lee, YS; Jeong, JM; Kim, WJ; Lee, DS
- Date Issued
- 2011-07
- Publisher
- ELSEVIER SCI LTD
- Abstract
- Recent advances in efficient microRNA (miRNA) delivery techniques using brain-targeted nanoparticles offer critical information for understanding the functional role of miRNAs in vivo, and for supporting targeted gene therapy in terms of treating miRNA-associated neurological diseases. Here, we report the rabies virus glycoprotein (RVG)-labeled non-toxic SSPEI nanomaterials capable of neuron-specific miR-124a delivery to neuron in vivo. The RVG-labeled BPEI-SS (RVG-SSPEI) nanocarrier showed less toxicity in acetylcholine receptor-positive Neuro2a cells, and electrostatic interaction of RVG-SSPEI with miR-124a exhibited optimal transfection efficacy. The RVG-SSPEI polymer specifically targeted Neuro2a using cy5.5-miR-124a mixed with RVG-SSPEI. The functional action of miR-124a oligomers released from polyplexes in the cytoplasmic region was evaluated by a reporter vector containing a miR-124a -binding sequence, and showed a significantly reduced reporter signal in a dose-dependent manner. Cy5.5-miR-124a/RVG-SSPEI- injected into mice via tail veins displayed the enhanced accumulation of miR-124a in the isolated brain. Hindrance of the efficient penetration of neuronal cells by size limitation of the miR-124a/RVG-SSPEI improved with the help of mannitol through blood-brain barrier disruption. These findings indicated that the RVG peptide combined with mannitol infusion using SSPEI polymer for neuron-specific targeting in vivo is sufficient to deliver neurogenic microRNA into the brain. (C) 2011 Elsevier Ltd. All rights reserved.
- Keywords
- MicroRNA; RVG peptide; PEI polymer; Brain-targeting agent; Polyplex; CENTRAL-NERVOUS-SYSTEM; NEURONAL DIFFERENTIATION; RNA INTERFERENCE; SIRNA; NANOPARTICLES; MIR-124; VECTOR; EXPRESSION; TOXICITY; MICE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17353
- DOI
- 10.1016/J.BIOMATERIALS.2011.03.047
- ISSN
- 0142-9612
- Article Type
- Article
- Citation
- BIOMATERIALS, vol. 32, no. 21, page. 4968 - 4975, 2011-07
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- There are no files associated with this item.
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