Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress
SCIE
SCOPUS
- Title
- Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress
- Authors
- Terahara, K; Nochi, T; Yoshida, M; Takahashi, Y; Goto, Y; Hatai, H; Kurokawa, S; Jang, MH; Kweon, MN; Domino, SE; Hiroi, T; Yuki, Y; Tsunetsugu-Yokota, Y; Kobayashi, K; Kiyono, H
- Date Issued
- 2011-01-21
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer's patch M cells and F-ECs was distinctly regulated by alpha(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells. (C) 2010 Elsevier Inc. All rights reserved.
- Keywords
- Epithelial cell; Fucosyltransferase; Intestine; M cell; Peyer' s patch; FOLLICLE-ASSOCIATED EPITHELIUM; PATCH M-CELLS; PEYERS-PATCH; LYMPHOID FOLLICLES; EXPRESSION; MICE; TISSUE; DIFFERENTIATION; GLYCOLIPIDS; BINDING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17304
- DOI
- 10.1016/J.BBRC.2010.12.067
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 404, no. 3, page. 822 - 828, 2011-01-21
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- There are no files associated with this item.
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