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PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana SCIE SCOPUS

Title
PHOSPHATIDYLSERINE SYNTHASE1 is required for microspore development in Arabidopsis thaliana
Authors
Yamaoka, YYu, YBMizoi, JFujiki, YSaito, KNishijima, MLee, YNishida, I
Date Issued
2011-08
Publisher
WILEY-BLACKWELL
Abstract
Phosphatidylserine (PS) has many important biological roles, but little is known about its role in plants, partly because of its low abundance. We show here that PS is enriched in Arabidopsis floral tissues and that genetic disruption of PS biosynthesis decreased heterozygote fertility due to inhibition of pollen maturation. At1g15110, designated PSS1, encodes a base-exchange-type PS synthase. Escherichia coli cells expressing PSS1 accumulated PS in the presence of L-serine at 23 degrees C. Promoter-GUS assays showed PSS1 expression in developing anther pollen and tapetum. A few seeds with pss1-1 and pss1-2 knockout alleles escaped embryonic lethality but developed into sterile dwarf mutant plants. These plants contained no PS, verifying that PSS1 is essential for PS biosynthesis. Reciprocal crossing revealed reduced pss1 transmission via male gametophytes, predicting a rate of 61.6% pss1-1 pollen defects in PSS1/pss1-1 plants. Alexander's staining of inseparable qrt1-1 PSS1/pss1-1 quartets revealed a rate of 42% having three or four dead pollen grains, suggesting sporophytic pss1-1 cell death effects. Analysis with the nuclear stain 4', 6-diamidino-2-phenylindole (DAPI) showed that all tetrads from PSS1/pss1-1 anthers retain their nuclei, whereas unicellular microspores were sometimes anucleate. Transgenic Arabidopsis expressing a GFP-LactC2 construct that binds PS revealed vesicular staining in tetrads and bicellular microspores and nuclear membrane staining in unicellular microspores. Hence, distribution and/or transport of PS across membranes were dynamically regulated in pollen microspores. However, among unicellular microspores from PSS1/pss1-2 GFP-LactC2 plants, all anucleate microspores showed little GFP-LactC2 fluorescence, suggesting that pss1-2 microspores are more sensitive to sporophytic defects or show partial gametophytic defects.
Keywords
phosphatidylserine; cell death; microspores; Lact-C2; very-long-chain fatty acids; Arabidopsis; BINDING C2 DOMAIN; ENDOPLASMIC-RETICULUM; APOPTOTIC CELLS; PLANT-CELLS; ESCHERICHIA-COLI; MEMBRANE PHOSPHATIDYLSERINE; PHOSPHOLIPID-COMPOSITION; DIFFERENTIAL EXPRESSION; DECARBOXYLASE ACTIVITY; LIPID-COMPOSITION
URI
https://oasis.postech.ac.kr/handle/2014.oak/17133
DOI
10.1111/J.1365-313X.2011.04624.X
ISSN
0960-7412
Article Type
Article
Citation
PLANT JOURNAL, vol. 67, no. 4, page. 648 - 661, 2011-08
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이영숙LEE, YOUNGSOOK
Dept of Life Sciences
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