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Proteomic analysis of microvesicles derived from human colorectal cancer ascites

Title
Proteomic analysis of microvesicles derived from human colorectal cancer ascites
Authors
Choi, DSPark, JOJang, SCYoon, YJJung, JWChoi, DYKim, JWKang, JSPark, JHwang, DLee, KHPark, SHKim, YKDominic M DesiderioKim, KPGho, YS
POSTECH Authors
Park, JHwang, DKim, YKGho, YS
Date Issued
Jul-2011
Publisher
WILEY-BLACKWELL
Abstract
The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites-derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1-D SDS-PAGE and nano-LC-MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence
384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon-specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.
Keywords
Animal proteomics; Ascites; Colorectal cancer; Exosomes; Microvesicles; MEMBRANE-VESICLES; OVARIAN-CANCER; MALIGNANT ASCITES; CELL-LINES; TUMOR; ANGIOGENESIS; EXOSOMES; EXPRESSION; EFFUSIONS; CARCINOMA
URI
http://oasis.postech.ac.kr/handle/2014.oak/17128
DOI
10.1002/PMIC.201100022
ISSN
1615-9853
Article Type
Article
Citation
PROTEOMICS, vol. 11, no. 13, page. 2745 - 2751, 2011-07
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박재성PARK, JAE SUNG
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