A visible light-induced photocatalytic silver enhancement reaction for gravimetric biosensors
SCIE
SCOPUS
- Title
- A visible light-induced photocatalytic silver enhancement reaction for gravimetric biosensors
- Authors
- Ko, W; Yim, C; Jung, N; Joo, J; Jeon, S; Seo, H; Lee, SS; Park, JC
- Date Issued
- 2011-10-07
- Publisher
- IOP PUBLISHING LTD
- Abstract
- We have developed a novel microgravimetric immunosensor using a WO3 nanoparticle-modified immunoassay and a silver enhancement reaction. When the nanoparticles in silver ion solution (i.e. AgNO3) are exposed to visible light, the silver ions are photocatalytically reduced and form a metallic silver coating on the nanoparticles. This silver coating consequently induces changes in the mass and light absorption spectrum. Although photocatalytic reduction reactions can be achieved using ultraviolet (UV) light and TiO2 nanoparticles as described in our previous publication (Seo et al 2010 Nanotechnology 21 505502), the use of UV light in biosensing applications has drawbacks in that UV light can damage proteins. In addition, conventional quartz crystal substrates must be passivated to prevent undesirable silver ion reduction on their gold-coated sensing surfaces. We addressed these problems by adopting a visible light-induced photocatalytic silver enhancement method using WO3 nanoparticles and lateral field excited (LFE) quartz crystals. As a proof-of-concept demonstration of the technique, streptavidin was adsorbed onto an LFE quartz crystal, and its mass was enhanced with biotinylated WO3 nanoparticles, this being followed by a photocatalytic silver enhancement reaction. The mass change due to the enhancement was found to be >30 times greater than the mass change obtained with the streptavidin alone.
- Keywords
- STAINING ENHANCEMENT; NANOGOLD PROBE; NANOPARTICLES; IMMUNOASSAY; MICROARRAYS; SCATTERING; REDUCTION; ARRAY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/17037
- DOI
- 10.1088/0957-4484/22/40/405502
- ISSN
- 0957-4484
- Article Type
- Article
- Citation
- NANOTECHNOLOGY, vol. 22, no. 40, 2011-10-07
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- There are no files associated with this item.
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