Proteomic Analysis of Tumor Necrosis Factor-Alpha (TNF-alpha)-Induced L6 Myotube Secretome Reveals Novel TNF-alpha-Dependent Myokines in Diabetic Skeletal Muscle
SCIE
SCOPUS
- Title
- Proteomic Analysis of Tumor Necrosis Factor-Alpha (TNF-alpha)-Induced L6 Myotube Secretome Reveals Novel TNF-alpha-Dependent Myokines in Diabetic Skeletal Muscle
- Authors
- Yoon, JH; Song, P; Jang, JH; Kim, DK; Choi, S; Kim, J; Ghim, J; Kim, D; Park, S; Lee, H; Kwak, D; Yea, K; HWANG, DAEHEE; Suh, PG; Ryu, SH
- Date Issued
- 2011-12
- Publisher
- Washington, D.C. : American Chemical Society,
- Abstract
- There is a strong possibility that skeletal muscle can respond to irregular metabolic states by secreting specific cytokines. Obesity-related chronic inflammation, mediated by pro-inflammatory cytokines, is believed to be one of the causes of insulin resistance that results in type 2 diabetes. Here, we attempted to identify and characterize the members of the skeletal muscle secretome in response to tumor necrosis factor-alpha (TNF-alpha)-induced insulin resistance. To conduct this study, we comparatively analyzed the media levels of proteins released from L6 skeletal muscle cells. We found 28 TNF-a modulated secretory proteins by using separate filtering methods: Gene Ontology, SignalP, and SecretomeP, as well as the normalized Spectral Index for label-free quantification. Ten of these secretory proteins were increased and 18 secretory proteins were decreased by TNF-a treatment. Using microarray analysis of Zuker diabetic rat skeletal muscle combined with bioinformatics and QPCR, we found a correlation between TNF-alpha-mediated insulin resistance and type 2 diabetes. This novel approach combining analysis of the conditioned secretome and transcriptome has identified several previously unknown, TNF-alpha-dependent secretory proteins, which establish a foothold for research on the different causes of insulin resistance and their relationships with each other.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16969
- DOI
- 10.1021/PR200573B
- ISSN
- 1535-3893
- Article Type
- Article
- Citation
- JOURNAL OF PROTEOME RESEARCH, vol. 10, no. 12, page. 5315 - 5325, 2011-12
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