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Repair of rabbit ulna segmental bone defect using freshly isolated adipose-derived stromal vascular fraction SCIE SCOPUS

Title
Repair of rabbit ulna segmental bone defect using freshly isolated adipose-derived stromal vascular fraction
Authors
Kim, AKim, DHSong, HRKang, WHKim, HJLim, HCCho, DWBae, JH
Date Issued
2012-03
Publisher
Informa Healthcare
Abstract
Background aims. Stromal vascular fractions (SVF) from adipose tissue have heterogeneous cell populations, and include multipotent adipose-derived stem cells. The advantages of using of SVF include the avoidance of an additional culture period, a reduced risk of extensive cell contamination, and cost-effectiveness. Methods. Unilateral 20-mm mid-diaphyseal segmental defects in rabbit ulna were treated with one of the following: polylactic glycolic acid (PLGA) scaffold alone (group 1, control), a PLGA scaffold with undifferentiated SVF cells (group 2), or a PLGA scaffold with osteogenically differentiated SVF cells (group 3). At 8 weeks after implantation, five rabbits in each treatment group were killed to assess bone defect healing by plain radiography, quantitative microcomputed tomography and histology. Results. The SVF cells were well grown on PLGA scaffolds and expressed type I collagen and alkaline phosphatase (ALP). The intensity of ALP and OPN gene expressions in osteogenic medium culture were increased from 14 days to 28 days. In vivo evaluations at 8 weeks showed that treatment of SVF cells with or without osteogenic differentiation resulted in more bone formation in the critically sized segmental defects than PLGA scaffold alone. Osteogenically differentiated SVF cells significantly enhanced bone healing compared with undifferentiated SVF cells. Conclusions. Adipose-derived stromal SVF showed osteogenic potential in vitro. Accordingly, SVF could provide a cell source for bone tissue engineering. However, treatment with uncultured SVF cells on bone healing was not satisfactory in the in vivo animal model.
Keywords
adipose tissue; bone; mesenchymal stromal cell; stromal vascular fraction; tissue engineering; ISOLATED FAT CELLS; PERFRINGENS ALPHA TOXIN; MESENCHYMAL STEM-CELLS; IN-VIVO; LIPOLYTIC HORMONES; UMBILICAL-CORD; TISSUE; SCAFFOLD; METABOLISM; INSULIN
URI
https://oasis.postech.ac.kr/handle/2014.oak/16630
DOI
10.3109/14653249.2011.627915
ISSN
1465-3249
Article Type
Article
Citation
CYTOTHERAPY, vol. 14, no. 3, page. 296 - 305, 2012-03
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조동우CHO, DONG WOO
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