Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds
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- Title
- Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds
- Authors
- Lim Chae-Oh; Lee Soo-In; Chung Woo-Sik; Park Sung-Han; Hwang, I; Cho Moo-Je
- Date Issued
- 1996-01
- Publisher
- Plant Molecular Biology
- Abstract
- A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
- Keywords
- Chinese cabbage (Brassica campestris L spp pekinensis); cDNA; cystatin; inhibitor; gene expression; CYSTATIN SUPERFAMILY MEMBER; EGG-WHITE CYSTATIN; MOLECULAR-CLONING; SEQUENCE-ANALYSIS; ANIMAL CYSTATINS; ORYZACYSTATIN-II; PLANT-ORIGIN; RICE SEEDS; EXPRESSION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16473
- DOI
- 10.1007/BF00020124
- ISSN
- 0167-4412
- Article Type
- Article
- Citation
- PLANT MOLECULAR BIOLOGY, vol. 30, no. 2, page. 373 - 379, 1996-01
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