Sequence Analysis and Expression of Met-rich Storage Protein SP-1 of Hyphantria cunea
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SCOPUS
- Title
- Sequence Analysis and Expression of Met-rich Storage Protein SP-1 of Hyphantria cunea
- Authors
- Chung Hyang Mi; Hwan, HI; Hwa, CD; Jae, SS
- Date Issued
- 1998-04-30
- Publisher
- Korean Society for Molecular and Cellular Biology
- Abstract
- We isolated and sequenced a cDNA clone corresponding to the 2.5 kb storage protein (SP-1) from fall webworm, Hyphantria cunea, The SP-1 gene encoded a pre-protein of 753 amino acids, including a signal peptide of 15 amino acids. The deduced amino acid sequence of SP-1 contained one potential N-glycosylation site, and the calculated isoelectric point and molecular weight of secreted SP-1 were pi = 8.38 and 86.8 kDa, respectively. A Northern blot of mRNA from various developmental stages revealed that the SP-1 mRNA in fat body appears in early last instar larvae and accumulates to a maximum level at the end of last instar larvae. The persistence of SP-1 transcript through the early pupal stage suggests that its mRNA might be stable or expressed during the pupal stage. SP-1 transcription was also found in the ovary as well as testis, This local expression of SP-1 in both reproductive organs seems to allow the insect to keep its reproductive activity under a nutritional stress.
- Keywords
- Hyphantria cunea; met-rich storage protein; ovary; storage protein; CALPODES-ETHLIUS LEPIDOPTERA; LARVAL SERUM-PROTEIN; GALLERIA-MELLONELLA; HEMOLYMPH-PROTEINS; BOMBYX-MORI; NUCLEOTIDE-SEQUENCE; MANDUCA-SEXTA; DEVELOPMENTAL REGULATION; DROSOPHILA-MELANOGASTER; MOLECULAR-CLONING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16466
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 8, no. 2, page. 219 - 225, 1998-04-30
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