Two type I crustacean hyperglycemic hormone (CHH) genes in Morotoge shrimp (Pandalopsis japonica): Cloning and expression of eyestalk and pericardial organ isoforms produced by alternative splicing and a novel type I CHH with predicted structure shared wi
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- Title
- Two type I crustacean hyperglycemic hormone (CHH) genes in Morotoge shrimp (Pandalopsis japonica): Cloning and expression of eyestalk and pericardial organ isoforms produced by alternative splicing and a novel type I CHH with predicted structure shared wi
- Authors
- Jeon, JM; Kim, BK; Lee, JH; Kim, HJ; Kang, CK; Mykles, DL; Kim, HW
- Date Issued
- 2012-08
- Publisher
- ELSEVIER SCIENCE INC
- Abstract
- Crustacean hyperglycemic hormone (CHH) peptide family members play critical roles in growth and reproduction in decapods. Three cDNAs encoding CHH family members (Pj-CHH1ES, Pj-CHH1PO, and Pj-CHH2) were isolated by a combination of bioinformatic analysis and conventional cloning strategies. Pj-CHH1ES and Pj-CHH1PO were products of the same gene that were generated by alternative mRNA splicing, whereas Pj-CHH2 was the product of a second gene. The Pj-CHH1 and Pj-CHH2 genes had four exons and three introns, suggesting the two genes arose from gene duplication. The three cDNAs were classified in the type I CHH subfamily, as the deduced amino acid sequences had a CHH precursor-related peptide sequence positioned between the N-terminal signal sequence and C-terminal mature peptide sequence. The Pj-CHH1ES isoform was expressed at a higher level in the eyestalk X-organ/sinus gland (XO/SG) complex and at a lower level in the gill. The Pj-CHH1PO isoform was expressed at higher levels in the XO/SG complex, brain, abdominal ganglion, and thoracic ganglion and at a lower level in the epidermis. Pj-CHH2 was expressed at a higher level in the thoracic ganglion and at a lower level in the gill. Real-time polymerase chain reaction was used to quantify the effects of eyestalk ablation on the mRNA levels of the three Pj-CHHs in the brain, thoracic ganglion, and gill. Eyestalk ablation reduced expression of Pj-CHH1ES in the brain and Pj-CHH1PO and Pj-CHH2 in the thoracic ganglion. Sequence alignment of the Pj-CHHs with CHHs from other species indicated that Pj-CHH2 had an additional alanine at position #9 of the mature peptide. Molecular modeling showed that the Pj-CHH2 mature peptide had a short alpha helix (alpha 1) in the N-terminal region, which is characteristic of type II CHHs. This suggests that Pj-CHH2 differs in function from other type 1 CHHs. (C) 2012 Elsevier Inc. All rights reserved.
- Keywords
- Crustacean hyperglycemic hormone (CHH); Pandalopsis japonica; Neuropeptide; Crustacean; Gene expression; MOLT-INHIBITING HORMONE; PRAWN MARSUPENAEUS-JAPONICUS; CRAYFISH PROCAMBARUS-CLARKII; PRECURSOR-RELATED PEPTIDES; AMINO-ACID-SEQUENCES; TROPICAL LAND CRAB; KURUMA PRAWN; CARCINUS-MAENAS; DECAPOD CRUSTACEANS; PENAEUS-JAPONICUS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16418
- DOI
- 10.1016/J.CBPB.2012.
- ISSN
- 1096-4959
- Article Type
- Article
- Citation
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, vol. 162, no. 4, page. 88 - 89, 2012-08
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