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Cited 3 time in webofscience Cited 4 time in scopus
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Simultaneous Detection of 10 Foodborne Pathogens using Capillary Electrophoresis-Based Single Strand Conformation Polymorphism SCIE SCOPUS KCI

Title
Simultaneous Detection of 10 Foodborne Pathogens using Capillary Electrophoresis-Based Single Strand Conformation Polymorphism
Authors
Oh, MHHwang, HSChung, BPaik, HDHan, SKang, SMHam, JSKim, HWSeol, KHJang, AJung, GY
Date Issued
2012-04
Publisher
KOREAN SOC FOOD SCIENCE ANIMAL RESOURCES
Abstract
This report outlines the development of a rapid, simple, and sensitive detection system for pathogenic bacteria using a capillary electrophoresis-based, single strand conformation polymorphism (CE-SSCP) combined with PCR. We demonstrate that this method, used with primers targeting the V4 region of the 16S rRNA gene, is capable of the simultaneous detection of 10 microbes that could be associated with foodborne illness, caused by animal-derived foods: Salmonella enterica, Listeria monocytogenes, Escherichia coli O157:H7, Campylobacter jejuni, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Yersinia enterocolitica, Vibrio parahaemolyticus, and Enterobacter sakazakii. The traditional detection techniques are time-consuming and labor-intensive, due to the necessary task of separate cultivation of each target species. As such, the CE-SSCP-PCR method, that we have developed, has the potential to diagnose pathogens rapidly, unlike the traditional technique, in order to prevent foodborne illness in a much more efficient manner.
Keywords
CE; SSCP; PCR; simultaneous detection; foodborne pathogens; RIBOSOMAL-RNA GENE; IDENTIFICATION; BACTERIA; PCR; FOOD
URI
https://oasis.postech.ac.kr/handle/2014.oak/16343
DOI
10.5851/KOSFA.2012.32.2.241
ISSN
1225-8563
Article Type
Article
Citation
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, vol. 32, no. 2, page. 241 - 246, 2012-04
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정규열JUNG, GYOO YEOL
Dept. of Chemical Enginrg
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