Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis
SCIE
SCOPUS
- Title
- Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis
- Authors
- Chung, B; Shin, GW; Hwang, HS; Chung, YJ; Jung, SW; Jung, GY
- Date Issued
- 2012-05-01
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection. (c) 2012 Elsevier Inc. All rights reserved.
- Keywords
- Swine H1N1 influenza; CE-SSCP; MLPA; Multiplex detection; Diagnosis; PATHOGENS; SYSTEM; VIRUS; MLPA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16341
- DOI
- 10.1016/J.AB.2012.02.009
- ISSN
- 0003-2697
- Article Type
- Article
- Citation
- ANALYTICAL BIOCHEMISTRY, vol. 424, no. 1, page. 54 - 56, 2012-05-01
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