Self-assembled complex of probe peptide - E. Coli RNA I conjugate and nano graphene oxide for apoptosis diagnosis
SCIE
SCOPUS
- Title
- Self-assembled complex of probe peptide - E. Coli RNA I conjugate and nano graphene oxide for apoptosis diagnosis
- Authors
- Kong, WH; Sung, DK; Kim, KS; Jung, HS; Gho, EJ; Yun, SH; Hahn, SK
- Date Issued
- 2012-10
- Publisher
- ELSEVIER SCI LTD
- Abstract
- Caspase-3 plays an important role in the initiation and propagation of apoptosis which is involved in various kinds of diseases including neurodegenerative diseases and inflammatory diseases. The capase-3 cleavable site of Asp-Glu-Val-Asp (DEVD) connected to a partial sequence of Escherichia coli RNAI was labeled with tetramethyl-6-carboxyrhodamine (TAMRA) as an optical probe. Graphene oxide (GO) was synthesized by a modified Hummer's method and exploited for the preparation of nano-sized GO (NGO) conjugated with polyethylene glycol (PEG). After binding the NGO-PEG by pi-pi stacking, the quenched fluorescence of TAMRA-DEVD-single stranded DNA (ssDNA) conjugate was recovered via the enzymatic cleavage by caspase-3 in live A549 cells. The comparative study with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay clearly confirmed the specific detection of apoptosis by the non-covalent TAMRA-DEVD-ssDNA/NGO-PEG complex. Furthermore, the self-assembled NGO complex was successfully exploited for in vivo diagnosis of apoptosis-related diseases like hypoxic-ischemic encephalopathy (HIE) and liver cirrhosis. (C) 2012 Elsevier Ltd. All rights reserved.
- Keywords
- Graphene oxide; Caspase; pi-pi stacking; Apoptosis; Diagnosis; RESONANCE ENERGY-TRANSFER; LIVE CELLS; CASPASE-3 ACTIVATION; REAL-TIME; DNA; ISCHEMIA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16278
- DOI
- 10.1016/J.BIOMATERIALS.2012.06.086
- ISSN
- 0142-9612
- Article Type
- Article
- Citation
- BIOMATERIALS, vol. 33, no. 30, page. 7556 - 7564, 2012-10
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