Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-Like Silica for Biodiesel Production
SCIE
SCOPUS
- Title
- Highly Efficient Enzyme Immobilization and Stabilization within Meso-Structured Onion-Like Silica for Biodiesel Production
- Authors
- Jun, SH; Lee, J; Kim, BC; Lee, JE; Joo, J; Park, H; Lee, JH; Lee, SM; Lee, D; Kim, S; Koo, YM; Shin, CH; Kim, SW; Hyeon, T; Kim, J
- Date Issued
- 2012-03-13
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Meso-structured onion-like silica (Meso-Onion-S) was synthesized and used as a host of enzyme immobilization. Meso-Onion-S has a 200-300 nm sized primary meso-structured onion building unit, and each onion unit has highly curved mesopores of 10 nm diameter in a multishell structure. Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a two-step process of enzyme adsorption and subsequent enzyme cross-linking, which effectively prevents the leaching of cross-linked enzyme aggregates from highly curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S significantly improved the enzyme stability as well as the enzyme loading. For example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days, while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking showed a rapid inactivation due to rigorous enzyme leaching under shaking. Stable NER-LP was successfully employed to produce biodiesels and fatty acid methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NER-LP was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in the form of NER-LP in the presence of organic solvents.
- Keywords
- meso-structured onion-like silica; enzyme immobilization; enzyme stabilization; biodiesel production; MESOPOROUS SILICA; CATALYTIC ACTIVITY; MOLECULAR-SIEVE; LIPASE; ACID; ENCAPSULATION; MORPHOLOGIES; COPOLYMER; VESICLES; TRIBLOCK
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16095
- DOI
- 10.1021/CM202125Q
- ISSN
- 0897-4756
- Article Type
- Article
- Citation
- CHEMISTRY OF MATERIALS, vol. 24, no. 5, page. 924 - 929, 2012-03-13
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