Synthetic RNA devices to expedite the evolution of metabolite-producing microbes
SCIE
SCOPUS
- Title
- Synthetic RNA devices to expedite the evolution of metabolite-producing microbes
- Authors
- Yang, J; Seo, SW; Jang, S; Shin, SI; Lim, CH; Roh, TY; Jung, GY
- Date Issued
- 2013-01
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- An extension of directed evolution strategies to genome-wide variations increases the chance of obtaining metabolite-overproducing microbes. However, a general high-throughput screening platform for selecting improved strains remains out of reach. Here, to expedite the evolution of metabolite-producing microbes, we utilize synthetic RNA devices comprising a riboswitch and a selection module that specifically sense inconspicuous metabolites. Using L-lysine-producing Escherichia coli as a model system, we demonstrated that this RNA device could enrich pathway-optimized strains to up to 75% of the total population after four rounds of enrichment cycles. Furthermore, the potential applicability of this device was examined by successfully extending its application to the case of L-tryptophan. When used in conjunction with combinatorial mutagenesis for metabolite overproduction, our synthetic RNA device should facilitate strain improvement.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16040
- DOI
- 10.1038/NCOMMS2404
- ISSN
- 2041-1723
- Article Type
- Article
- Citation
- NATURE COMMUNICATIONS, vol. 4, 2013-01
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