Turn-On Fluorescence Detection of Apoptotic Cells Using a Zinc(II)-Dipicolylamine-Functionalized Poly(diacetylene) Liposome
SCIE
SCOPUS
- Title
- Turn-On Fluorescence Detection of Apoptotic Cells Using a Zinc(II)-Dipicolylamine-Functionalized Poly(diacetylene) Liposome
- Authors
- Cho, YS; Kim, KM; Lee, D; Kim, WJ; Ahn, KH
- Date Issued
- 2013-04
- Publisher
- WILEY-BLACKWELL
- Abstract
- A liposome-based fluorescence sensing system for apoptotic cells has been developed from stimuli-responsive poly(diacetylene)-liposomes for the first time. The combination of the liposome components, a phosphatidylserine-binding ZnII-dipicolylamine component and an alcohol-terminated component in the ratio of 2:1, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn-on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for always-on-type sensing systems.
- Keywords
- apoptosis; fluorescence; liposome; phosphatidylserine; zinc; CONJUGATED POLYMERS; SELECTIVE AFFINITY; PHOSPHATIDYLSERINE; POLYDIACETYLENES; SUPRAMOLECULES; CHEMOSENSORS; COMPLEXES; RESPONSES; DEATH
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15496
- DOI
- 10.1002/ASIA.201201139
- ISSN
- 1861-4728
- Article Type
- Article
- Citation
- CHEMISTRY-AN ASIAN JOURNAL, vol. 8, no. 4, page. 755 - 759, 2013-04
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- There are no files associated with this item.
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