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Cited 4 time in webofscience Cited 3 time in scopus
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Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

Title
Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins
Authors
Jeong, HSChoi, SMKim, HWPark, JWPark, HNPark, SMJang, SKRhee, YMKim, BH
POSTECH Authors
Jang, SKRhee, YM
Date Issued
2013-05
Publisher
Royal Society of Chemistry
Abstract
This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
URI
https://oasis.postech.ac.kr/handle/2014.oak/15327
DOI
10.1039/C2MB25470K
ISSN
1742-206X
Article Type
Article
Citation
Molecular BioSystems, vol. 09, no. 5, page. 948 - 951, 2013-05
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