In Vivo Residue-Specific Dopa-Incorporated Engineered Mussel Bioglue with Enhanced Adhesion and Water Resistance
SCIE
SCOPUS
- Title
- In Vivo Residue-Specific Dopa-Incorporated Engineered Mussel Bioglue with Enhanced Adhesion and Water Resistance
- Authors
- Yang, B; Ayyadurai, N; Yun, H; Choi, YS; Hwang, BH; Huang, J; Lu, QY; Zeng, HB; Cha, HJ
- Date Issued
- 2014-12-01
- Publisher
- WILEY-VCH
- Abstract
- Misaminoacylation of 3,4-dihydroxyphenylalanine (Dopa) molecules to tRNATyr by endogenous tyrosyl-tRNA synthetase allowed the quantitative replacement of tyrosine residues with a yield of over 90% by an in vivo residue-specific incorporation strategy, to create, for the first time, engineered mussel adhesive proteins (MAPs) in Escherichia coli with a very high Dopa content, close to that of natural MAPs. The Dopa-incorporated MAPs exhibited a superior surface adhesion and water resistance ability by assistance of Dopa-mediated interactions including the oxidative Dopa crosslinking, and furthermore, showed underwater adhesive properties comparable to those of natural MAPs. These results propose promising use of Dopa-incorporated engineered MAPs as bioglues or adhesive hydrogels for practical underwater applications.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/14240
- DOI
- 10.1002/ANIE.201406099
- ISSN
- 1433-7851
- Article Type
- Article
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, vol. 53, no. 49, page. 13360 - 13364, 2014-12-01
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