Multiplex ligase-based genotyping methods combined with CE
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- Title
- Multiplex ligase-based genotyping methods combined with CE
- Authors
- Shin, GW; Chung, B; Jung, GY; Jung, GY
- Date Issued
- 2014-04
- Publisher
- WILEY-BLACKWELL
- Abstract
- In this genomic era, the ability to assay multiple genomic hot spots that have strong clinical implications is greatly desired. Conventional PCR-based methods suffer from frequent false-positive detections, particularly when a multiplex analysis is desirable. As an alternative to the error-prone conventional methods, multiplex ligase-based genotyping methods combined with CE have a strong potential. In this review, both previously developed methods and emerging methods are described to reveal the specificity, sensitivity, and simplicity of the ligase-based methods. For each step (ligation, amplification, and separation), the principles of several alternative methods are discussed along with their applications to explore the future development of ligase-based diagnostic methods.
- Keywords
- CE; Diagnostic methods; Genetic polymorphisms; Ligase-based genotyping; Multiplex analysis; DEPENDENT PROBE AMPLIFICATION; ROLLING CIRCLE AMPLIFICATION; SENSITIVE CAPILLARY-ELECTROPHORESIS; SOLUTION CONJUGATE ELECTROPHORESIS; MOLECULAR INVERSION PROBES; COPY NUMBER VARIATION; CHAIN-REACTION; DNA-LIGASE; MICROCHIP ELECTROPHORESIS; MUTATION DETECTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13917
- DOI
- 10.1002/ELPS.201300361
- ISSN
- 0173-0835
- Article Type
- Article
- Citation
- ELECTROPHORESIS, vol. 35, no. 7, page. 1004 - 1016, 2014-04
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