Bioimaging and pulmonary applications of self-assembled Flt1 peptide-hyaluronic acid conjugate nanoparticles
SCIE
SCOPUS
- Title
- Bioimaging and pulmonary applications of self-assembled Flt1 peptide-hyaluronic acid conjugate nanoparticles
- Authors
- Kim, H; Park, HT; Tae, YM; Kong, WH; Sung, DK; Hwang, BW; Kim, KS; Kim, YK; Hahn, SK
- Date Issued
- 2013-11
- Publisher
- ELSEVIER SCI LTD
- Abstract
- Despite wide exploitation of corticosteroid drugs for the treatment of asthma, the poor therapeutic effect on a neutrophilic subtype of asthma prohibits the full recovery of asthma patients. In this work, dexamethasone (Dexa) was loaded in Flt1 peptide-hyaluronic acid (HA) conjugate nanoparticles to overcome the limitation of corticosteroid resistance for the treatment of neutrophilic pulmonary inflammation. Fin peptide-HA conjugates are self-assembled to nanoparticles because of hydrophobic Flt1 peptide conjugated to HA by benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluorophosphate (BOP) chemistry. In vitro bioimaging showed efficient internalization of Fin peptide-HA conjugate nanoparticles into lung epithelial cells by HA-receptor mediated endocytosis. Also, ex vivo imaging for the biodistribution in ICR mice revealed long-term retention of Flt1 peptide-HA conjugate nanoparticles in deep lung tissues possibly due to mucoadhesive property of HA. On the basis of bioimaging results for pulmonary drug delivery applications, we prepared Dexa-loaded Flt1 peptide-HA conjugate nanoparticles. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) confirmed the formation of nanoparticles, which reduced cytokine levels of lipopolysaccharide (LPS)-stimulated cells more efficiently than free Dexa. Furthermore, according to the bronchoalveolar lavage (BAL) cellularity and histological analysis, Dexa loaded Flt1 peptide-HA conjugate nanoparticles showed remarkable therapeutic effects in both eosinophilic and neutrophilic asthma model mice. (C) 2013 Elsevier Ltd. All rights reserved.
- Keywords
- Hyaluronic acid; Flt1 peptide; Dexamethasone; Nanoparticle; Pulmonary delivery; Asthma; ENDOTHELIAL GROWTH-FACTOR; ANTI-FLT1 PEPTIDE; LUNG INJURY; ASTHMA; DELIVERY; RECEPTOR; TYPE-1; CELLS; NEOVASCULARIZATION; POLARIZATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13886
- DOI
- 10.1016/J.BIOMATERIALS.2013.07.062
- ISSN
- 0142-9612
- Article Type
- Article
- Citation
- BIOMATERIALS, vol. 34, no. 33, page. 8478 - 8490, 2013-11
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.