Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
SCIE
SCOPUS
- Title
- Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells
- Authors
- Rodriguez, JA; Xu, R; Chen, CC; Huang, ZF; Jiang, HD; Chen, AL; Raines, KS; Pryor, A; Nam, D; Wiegart, L; Song, C; Madsen, A; Chushkin, Y; Zontone, F; Bradley, PJ; Miao, JW
- Date Issued
- 2015-09
- Publisher
- IUCr
- Abstract
- A structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 keV X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the threedimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. It is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13467
- DOI
- 10.1107/S205225251501235X
- ISSN
- 2052-2525
- Article Type
- Article
- Citation
- IUCRJ, vol. 2, no. 5, page. 575 - 583, 2015-09
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