Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy
SCIE
SCOPUS
- Title
- Analytic 3D imaging of mammalian nucleus at nanoscale using coherent x-rays and optical fluorescence microscopy
- Authors
- Song, CY; Takagi, M; Park, J; Xu, R; Gallagher-Jones, M; Imamoto, N; Ishikawa, T
- Date Issued
- 2014-09-02
- Publisher
- Cell press
- Abstract
- Despite the notable progress that has been made with nano-bio imaging probes, quantitative nanoscale imaging of multistructured specimens such as mammalian cells remains challenging due to their inherent structural complexity. Here, we successfully performed three-dimensional (3D) imaging of mammalian nuclei by combining coherent x-ray diffraction microscopy, explicitly visualizing nuclear substructures at several tens of nanometer resolution, and optical fluorescence microscopy, cross confirming the substructures with immunostaining. This demonstrates the successful application of coherent x-rays to obtain the 3D ultrastructure of mammalian nuclei and establishes a solid route to nanoscale imaging of complex specimens.
- Keywords
- FREE-ELECTRON LASER; DIFFRACTION MICROSCOPY; ATOMIC-RESOLUTION; TOMOGRAPHY; CELLS; ARCHITECTURE; DIMENSIONS; NUCLEOLUS; DYNAMICS; WHOLE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13298
- DOI
- 10.1016/J.BPJ.2014.07.028
- ISSN
- 0006-3495
- Article Type
- Article
- Citation
- BIOPHYSICAL JOURNAL, vol. 107, no. 5, page. 1074 - 1081, 2014-09-02
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