Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy
SCIE
SCOPUS
- Title
- Quantitative 3D imaging of whole, unstained cells by using X-ray diffraction microscopy
- Authors
- Jiang, HD; Song, CY; Chen, CC; Xu, R; Raines, KS; Fahimian, BP; Lu, CH; Lee, TK; Nakashima, A; Urano, J; Ishikawa, T; Tamanoi, F; Miao, JW
- Date Issued
- 2010-06-22
- Publisher
- NATL ACAD SCIENCES
- Abstract
- Microscopy has greatly advanced our understanding of biology. Although significant progress has recently been made in optical microscopy to break the diffraction-limit barrier, reliance of such techniques on fluorescent labeling technologies prohibits quantitative 3D imaging of the entire contents of cells. Cryoelectron microscopy can image pleomorphic structures at a resolution of 3-5 nm, but is only applicable to thin or sectioned specimens. Here, we report quantitative 3D imaging of a whole, unstained cell at a resolution of 50-60 nm by X-ray diffraction microscopy. We identified the 3D morphology and structure of cellular organelles including cell wall, vacuole, endoplasmic reticulum, mitochondria, granules, nucleus, and nucleolus inside a yeast spore cell. Furthermore, we observed a 3D structure protruding from the reconstructed yeast spore, suggesting the spore germination process. Using cryogenic technologies, a 3D resolution of 5-10 nm should be achievable by X-ray diffraction microscopy. This work hence paves a way for quantitative 3D imaging of a wide range of biological specimens at nanometer-scale resolutions that are too thick for electron microscopy.
- Keywords
- coherent diffractive imaging; equally sloped tomography; lensless imaging; iterative phase-retrieval algorithms; oversampling; SCHIZOSACCHAROMYCES-POMBE; PHASE RETRIEVAL; RESOLUTION; TOMOGRAPHY; ALGORITHMS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13274
- DOI
- 10.1073/PNAS.1000156107
- ISSN
- 0027-8424
- Article Type
- Article
- Citation
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 107, no. 25, page. 11234 - 11239, 2010-06-22
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