Quantitative imaging of single, unstained viruses with coherent X-rays
SCIE
SCOPUS
- Title
- Quantitative imaging of single, unstained viruses with coherent X-rays
- Authors
- Song, CY; Jiang, HD; Mancuso, A; Amirbekian, B; Peng, L; Sun, R; Shah, SS; Zhou, ZH; Ishikawa, T; Miao, JW
- Date Issued
- 2008-10-10
- Publisher
- AMER PHYSICAL SOC
- Abstract
- We report the recording and reconstruction of x-ray diffraction patterns from single, unstained viruses, for the first time. By separating the diffraction pattern of the virus particles from that of their surroundings, we performed quantitative and high-contrast imaging of a single virion. The structure of the viral capsid inside a virion was visualized. This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles. Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes.
- Keywords
- DIFFRACTION MICROSCOPY; RESOLUTION; CRYSTALLOGRAPHY; ALLOW
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13272
- DOI
- 10.1103/PHYSREVLETT.101.158101
- ISSN
- 0031-9007
- Article Type
- Article
- Citation
- PHYSICAL REVIEW LETTERS, vol. 101, no. 15, page. 158101, 2008-10-10
- Files in This Item:
-
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.