Imaging a specific mRNA in pollen with atomic force microscopy
SCIE
SCOPUS
- Title
- Imaging a specific mRNA in pollen with atomic force microscopy
- Authors
- Kim, JS; Park, YS; Nam, HG; Park, JW
- Date Issued
- 2015-01
- Publisher
- Royal Society of Chemistry
- Abstract
- Intracellular localization of RNA is important in regulating various cellular functions. Here, we determined the intracellular localization of the ammonium transporter (AtAMT1; 4) mRNA in the sperm cells of Arabidopsis pollen by atomic force microscopy (AFM). The AFM probe was modified with cone-shaped dendron molecules, and an oligonucleotide complementary to the mRNA was attached to the apex. This configuration allowed facile detection of the mRNA within a sectioned pollen cell at the single-molecule level by measuring the mRNA-oligonucleotide interaction force. The AtAMT1; 4 mRNA was largely localized in the nucleus rather than the cytosol of the sperm cells.
- Keywords
- ARABIDOPSIS-THALIANA; GENE-EXPRESSION; IN-VIVO; LOCALIZATION; MOLECULES; BACTERIA; LEVEL; CELLS; TRANSCRIPTION; SPECTROSCOPY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13056
- DOI
- 10.1039/C5RA00199D
- ISSN
- 2046-2069
- Article Type
- Article
- Citation
- RSC ADVANCES, vol. 5, no. 24, page. 18858 - 18865, 2015-01
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