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A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization

Title
A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization
Authors
Shrestha, RChoi, TYChang, WKIM, DONG SIK
POSTECH Authors
KIM, DONG SIK
Date Issued
Sep-2011
Publisher
MDPI
Abstract
We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of similar to 2 mu m with an accuracy of +/- 0.01 degrees C. We produced and tested various micrometer-sized sensors, ranging from 2 mu m to 30 mu m. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of +/- 0.01 degrees C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 mu V/degrees C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 mu m. We measured transient temperature profiles and the maximum temperatures were in the range of 38-55 +/- 0.5 degrees C.
We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of similar to 2 mu m with an accuracy of +/- 0.01 degrees C. We produced and tested various micrometer-sized sensors, ranging from 2 mu m to 30 mu m. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of +/- 0.01 degrees C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 mu V/degrees C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 mu m. We measured transient temperature profiles and the maximum temperatures were in the range of 38-55 +/- 0.5 degrees C.
URI
http://oasis.postech.ac.kr/handle/2014.oak/12914
DOI
10.3390/S110908826
ISSN
1424-8220
Article Type
Article
Citation
SENSORS, vol. 11, no. 9, page. 8826 - 8835, 2011-09
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김동식KIM, DONG SIK
Dept of Mechanical Enginrg
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