eIF4GI Facilitates the MicroRNA-Mediated Gene Silencing
SCIE
SCOPUS
- Title
- eIF4GI Facilitates the MicroRNA-Mediated Gene Silencing
- Authors
- Ryu, I; Park, JH; An, S; Kwon, OS; Jang, SK
- Date Issued
- 2013-02-07
- Publisher
- PUBLIC LIBRARY SCIENCE
- Abstract
- MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes to the mRNAs. However, the molecular basis of this silencing is unclear. Here, we show that human Ago2 associates with the cap-binding protein complex and this association is mediated by human eIF4GI, a scaffold protein required for the translation initiation. Using a cap photo-crosslinking method, we show that Ago2 closely associates with the cap structure. Taken together, these data suggest that eIF4GI participates in the miRNA-mediated post-transcriptional gene silencing by promoting the association of Ago2 with the cap-binding complex.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/12692
- DOI
- 10.1371/JOURNAL.PONE.0055725
- ISSN
- 1932-6203
- Article Type
- Article
- Citation
- PLOS ONE, vol. 8, no. 2, 2013-02-07
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