HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana
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SCOPUS
- Title
- HIGLE is a bifunctional homing endonuclease that directly interacts with HYL1 and SERRATE in Arabidopsis thaliana
- Authors
- Cho Seok Keun; Ryu Moon Young; Poulsen Christian; Kim Jong Hum; Oh Tae Rin; Choi Suk Won; Kim Mijung; Yang Jun-Yi; Boo Kyung Hwan; Geshi Naomi; Kim Woo Taek; Yang Seong Wook
- Date Issued
- 2017-05
- Publisher
- Elsevier BV
- Abstract
- A highly coordinated complex known as the microprocessor precisely processes primary transcripts of MIRNA genes into mature miRNAs. In plants, the microprocessor minimally consists of three components: Dicer-like protein 1 (DCL1), HYPONASTIC LEAF 1 (HYL1), and SERRATE (SE). To precisely modulate miRNA maturation, the microprocessor cooperates with at least 12 proteins in plants. In addition, we here show the involvement of a novel gene, HYL1-interacting GIY-YIG-like endonuclease (HIGLE). The encoded protein has a GIY-YIG domain that is generally found within a class of homing endonucleases. HIGLE directly interacts with the microprocessor components HYL1 and SE. Unlike the functions of other GIY-YIG endonucleases, the catalytic core of HIGLE has both DNase and RNase activities that sufficiently processes miRNA precursors into short fragments in vitro.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/118173
- DOI
- 10.1002/1873-3468.12628
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS Letters, vol. 591, no. 10, page. 1383 - 1393, 2017-05
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