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Cited 34 time in webofscience Cited 35 time in scopus
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Contribution of RNA polymerase concentration variation to protein expression noise SCIE SCOPUS

Title
Contribution of RNA polymerase concentration variation to protein expression noise
Authors
Yang, SKim, SLim, YRKim, CAn, HJKim, JHSung, JLee, NK
Date Issued
2014-09
Publisher
NATURE PUBLISHING GROUP
Abstract
Cell-to-cell variation in gene expression, or noise, is a general phenomenon observed within cell populations. Transcription is known to be the key stage of gene expression where noise is generated, however, how variation in RNA polymerase (RNAP) concentration contributes to gene expression noise is unclear. Here, we quantitatively investigate how variations in absolute amounts of RNAP molecules affect noise in the expression of two fluorescent protein reporters driven by identical promoters. We find that intrinsic noise is independent of variation in RNAP concentrations, whereas extrinsic noise, which is variation in gene expression due to varying cellular environments, scales linearly with variation in RNAP abundance. Specifically, the propagation of RNAP abundance variation to expressed protein noise is inversely proportional to the concentration of RNAP, which suggests that the change in noise that results from RNAP fluctuations is determined by the fraction of promoters that is not occupied by RNAP.
URI
https://oasis.postech.ac.kr/handle/2014.oak/11789
DOI
10.1038/NCOMMS5761
ISSN
2041-1723
Article Type
Article
Citation
NATURE COMMUNICATIONS, vol. 5, page. 4761, 2014-09
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