Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2
SCIE
SCOPUS
- Title
- Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2
- Authors
- Park, HS; Lee, SH; Park, D; Lee, JS; Ryu, SH; Lee, WJ; Rhee, SG; Bae, YS
- Date Issued
- 2004-05
- Publisher
- AMER SOC MICROBIOLOGY
- Abstract
- The generation of reactive oxygen species (ROS) in cells stimulated with growth factors requires the activation of phosphatidylinositol 3-kinase (PI3K) and the Rac protein. We report here that the COOH-terminal region of Nox1, a protein related to gp91(phox) (Nox2) of phagocytic cells, is constitutively associated with PPix, a guanine nucleotide exchange factor for Rac. Both growth factor-induced ROS production and Rac1 activation were completely blocked in cells depleted of betaPix by RNA interference. Rac1 was also shown to bind to the COOH-terminal region of Nox1 in a growth factor-dependent manner. Moreover, the depletion of Nox1 by RNA interference inhibited growth factor-induced ROS generation. These results suggest that ROS production in growth factor-stimulated cells is mediated by the sequential activation of PI3K, betaPix, and Rac1, which then binds to Nox1 to stimulate its NADPH oxidase activity.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/11683
- DOI
- 10.1128/MCB.24.10.4384-4394.2004
- ISSN
- 0270-7306
- Article Type
- Article
- Citation
- MOLECULAR AND CELLULAR BIOLOGY, vol. 24, no. 10, page. 4384 - 4394, 2004-05
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