Study on controllable enzymolysis by chiral capillary electrophoresis with an ultraviolet-visible responsive polymer membrane based L-asparaginase reactor
SCIE
SCOPUS
- Title
- Study on controllable enzymolysis by chiral capillary electrophoresis with an ultraviolet-visible responsive polymer membrane based L-asparaginase reactor
- Authors
- Shen, Ji; Qiao, Juan; Kim, Dong-Pyo; Qi, Li
- Date Issued
- 2021-11
- Publisher
- ELSEVIER
- Abstract
- Stimuli-responsive polymer enzyme reactors have become a major area of research interest, from their fundamental aspects to applications in bio-living science. However, the polymer materials, that enable the controllable enzymolysis based on ultraviolet-visible (UV)-responsive properties, have remained unexplored. Herein, an enzyme reactor was fabricated by immobilization of L-asparaginase on an UV-responsive porous polymer membrane (UV-PPMER), which consisted of poly(styrene-maleicanhydride-4-[(4-methacryloyloxy)phenylazo] benzoic acid) [P(St-MAn-MPABA)], and explored its controllable enzymolysis. By controlling the "on/off" switch of 365 nm UV irradiation, the configuration of polymer membrane surface changed to improve and tune the enzymolysis. Using L-asparagine (L-Asn) as the substrate, the enzymatic efficiency of the UV-PPMER was evaluated by a chiral capillary electrophoresis technique. Upon UV irradiation, the PMPABA moiety in the membrane changed from a trans- to a cisconfiguration and encapsulated the enzyme and substrate into a narrow cavity, further improving the enzymatic efficiency due to the confinement effect. It was found that the enzymatic reaction rate with the UV-PPMER under UV irradiation (13.3 mM min(-1)) was 4.5 times higher than that of UV irradiation was off (2.94 mM min(-1)). Additionally, the low cytotoxicity and excellent UV-responsivity of UV-PPMER were verified in the living cells and serum samples.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/113067
- DOI
- 10.1016/j.talanta.2021.122676
- ISSN
- 0039-9140
- Article Type
- Article
- Citation
- TALANTA, vol. 234, 2021-11
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- There are no files associated with this item.
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